FastQCFastQC Report
Sun 21 May 2023
EGAF00007924580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487720
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT119372.447510866890839No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG55941.1469695727056508No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40050.8211678832116789No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG28820.5909128188304764No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA28120.5765603214959403No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC21080.4322152054457476No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC20030.4106864594439433No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16880.3461002214385303No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC16720.34281965061920777No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC16150.3311326170753711No Hit
CAGCCGCAGCGCCGTAAACGTCGTGCGGCTC15350.3147297629787583No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC13930.28561469695727054No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC13060.26777659312720414No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT12830.26306077257442795No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12070.2474780611826458No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11430.23435577790535553No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11120.22799967194291806No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC10900.22348888706634953No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10600.21733781678011976No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA10260.2103666037890593No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9420.19314360698761585No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9040.18535225129172475No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8910.18268678750102518No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA7690.15767243500369063No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA7200.1476256868695153No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6810.13962929549741654No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6670.1367587960305093No Hit
GCGGAGCAGCACACGCCGATGAAGGCGCACG6500.13327318953497908No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6430.13183793980152547No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6430.13183793980152547No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT6190.1269170835725416No Hit
GCACCGAGCGTTGATCCAATCGTGGAGGCTA6140.12589190519150334No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5920.1213811203149348No Hit
GCGCTGAAGGCGGGCGCAGTTGAAGCGGCGT5870.1203559419338965No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5660.11605019273353563No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5610.11502501435249735No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5590.114614943000082No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5500.11276962191421308No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5320.10907897974247519No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC5280.10825883703764455No Hit
GTTACCCTGGATGCAGGTAACCGTTTCTCTA5180.10620848027556795No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5060.10374805216107603No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5010.10272287378003772No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG4990.10231280242762242No Hit
GCTAAACTGAACCCGTCTAGCATCAAGGCCC4980.10210776675141475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)