FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609195
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGCAGATAGCGTTCTGCTTCGATTGCGGCGCTGCAACCCA177672.9164717372926567No Hit
GAAGCCGTAGTTGTAAAAGTCCCAAGAACCATATTTGGCGTGCA65611.0769950508457884No Hit
GATACCCGGGGTTACTTTGTCCAGAGCTTTGATCATTTCGTCGA52780.8663892513891283No Hit
TACGGTGCAGGTATAGTAAGAGTGGAAGAAGAGAAGCTCGGGCT50120.8227250716108964No Hit
ACCGGATACGTCGATAACCCACCATTTGTCACGGTGTTTGAAGT46980.7711816413463669No Hit
GAAGGCGGCGATGTCAGCAGTGGTAATCGCCTTGCCCGGCAGGG39520.6487249567051601No Hit
TTCTTTACCCATCTGGTCGCTGTCCGGGGTACGCACGTACAGCG38860.6378909872865011No Hit
GGACGGAGCTGGAGACGCGGCACGCAGGCTCGCATAACCACGGT30460.5000041037762949No Hit
TTCATCCGCGACAGCAGAGGCGGCAACGGAGTCTGCGGCAGAAT29000.4760380502138067No Hit
TTCTTTGGTGAAGCGGGTGACATAATCTGGGTCCACGTCGTACA18960.31123039420875087No Hit
ACGGAAGTCCCAGCCTTTCCACGCGGCGTCCAGGGTCAGGCCGA15400.25279261976871115No Hit
GCGTGCGGACACAATACCCAGCTGAGCGCCGTCCGGACCAATCA15190.249345447680956No Hit
GTCGGTCGGGCGGGCGTCAGCGTTTTTGACCCAGAAGAATTTCC14000.22981147251701015No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT12350.20272654897036252No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11720.1923850327070971No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC10620.174328417009332No Hit
CAGGGTGCCAAAGTCGGTCGGAGCGAAGCCCAGATCACCGATTT9650.15840576498493913No Hit
CTGTTCGATATCGGTCAGAAAGACGCTTTGGACATCTGGGTGGC8810.14461707663391854No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG8480.13920009192458901No Hit
ATACAGGGCGCGCGCACGGTCTTCATCGGACAGTTTCAGTTTCA8310.13640952404402532No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC8230.1350963156296424No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT7930.13017178407570645No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6930.11375667889592003No Hit
TTTGACGGAAATGCGACGTTTCTTGCTAACGAATACGATGCCTG6900.11326422574052643No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC6860.11260762153333498No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT6260.10275855842546311No Hit
CTGGACGGAAACAGAACGACGGCTACGTTTGGAGTGGCGGGTAC6190.10160950106287807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)