FastQCFastQC Report
Wed 31 May 2023
EGAF00007924622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609195
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTTTCCTGGGCGATCTGGTTGACCGTG172762.8358735708599054No Hit
TACAGGAAGGTGTCCTGACCTGGTACGAAGA64721.0623856072357785No Hit
AAGATTCTGCTGCAGCGTTACGGTGATTTCC52360.859494907213618No Hit
GCGCTGACGGCCACCTTCTCCAACATCGTGG49670.8153382742799925No Hit
ATGCACGTGATTTCTCGTAAACCGTTCAACG45760.7511552130270275No Hit
CGTACCGTAAAAGGTGGCACCATCGAGCCGC38290.6285343773340227No Hit
ATGCTGTGGGCTCGTCAGGTCATTTTCATGA37830.6209834289513211No Hit
GTTGGCGGCGTAGGCGCAGGCGCAGACCCGT30100.49409466591157186No Hit
TTCTCCAGTCAGTCTGGTAGCTGGTACCTGA28510.4679946486757114No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA25320.41563046315219265No Hit
TCTACTCTGATCCGCCTGATGTCGGTGTTGA18610.30548510739582563No Hit
CAGTCCTTTACCGAATGGCAGAACCCGGAGG15300.25115110925073253No Hit
GACCAGAGCACTCTGGGCTCTAATTGGGGTG15070.24737563505938162No Hit
ATGGACATCTGTCTGCGTTTCTTCTGCGCGG14900.24458506717881795No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA12590.2066661742135113No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11950.19616050689844794No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA11390.18696804799776756No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10090.16562841126404518No Hit
CCGGCGTCTAGCGGTTTCGCAAGCGTTATGC9340.15331708237920533No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG9200.15101896765403525No Hit
GACAGCATGCGTACCATCGATCAGACCTACA8700.14281141506414202No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG8640.14182650875335484No Hit
ACTATTCTGACCTACGCACCGAAAGCAATTG8140.13361895616346162No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7060.11589064256929227No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG6970.11441328310311148No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT6760.11096611101535632No Hit
GGTTCTTCCAAAGGTGACGTTGCAGAGGGTG6630.1088321473419841No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6420.10538497525422895No Hit
ATGTGCGAAGACACCATCACCTACGAATGTC6190.10160950106287807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)