FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592286
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGCAGATAGCGTTCTGCTTCGATTGCGGCGCTGCAACCCA171102.888807096571589No Hit
GAAGCCGTAGTTGTAAAAGTCCCAAGAACCATATTTGGCGTGCA63851.0780264939573112No Hit
GATACCCGGGGTTACTTTGTCCAGAGCTTTGATCATTTCGTCGA50410.8511090925667666No Hit
TACGGTGCAGGTATAGTAAGAGTGGAAGAAGAGAAGCTCGGGCT49960.8435114117166369No Hit
ACCGGATACGTCGATAACCCACCATTTGTCACGGTGTTTGAAGT45150.7623006452963602No Hit
GAAGGCGGCGATGTCAGCAGTGGTAATCGCCTTGCCCGGCAGGG38860.6561019507467676No Hit
TTCTTTACCCATCTGGTCGCTGTCCGGGGTACGCACGTACAGCG37280.6294256490952006No Hit
GGACGGAGCTGGAGACGCGGCACGCAGGCTCGCATAACCACGGT30610.5168111351610539No Hit
TTCATCCGCGACAGCAGAGGCGGCAACGGAGTCTGCGGCAGAAT28390.47932924296708007No Hit
TTCTTTGGTGAAGCGGGTGACATAATCTGGGTCCACGTCGTACA18840.3180895715921025No Hit
GCGTGCGGACACAATACCCAGCTGAGCGCCGTCCGGACCAATCA15510.2618667333011417No Hit
ACGGAAGTCCCAGCCTTTCCACGCGGCGTCCAGGGTCAGGCCGA14000.23637229311515043No Hit
GTCGGTCGGGCGGGCGTCAGCGTTTTTGACCCAGAAGAATTTCC13950.23552810635402494No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12010.20277366002235406No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT11990.20243598531790385No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC11260.190110858605471No Hit
CAGGGTGCCAAAGTCGGTCGGAGCGAAGCCCAGATCACCGATTT9290.15684990021712483No Hit
CTGTTCGATATCGGTCAGAAAGACGCTTTGGACATCTGGGTGGC8130.13726476735901236No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG8050.13591406854121152No Hit
ATACAGGGCGCGCGCACGGTCTTCATCGGACAGTTTCAGTTTCA8010.13523871913231109No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC8000.13506988178008597No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT7590.12814755033885655No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC7130.12038103213650163No Hit
TTTGACGGAAATGCGACGTTTCTTGCTAACGAATACGATGCCTG6830.1153159115697484No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6540.11041962835522028No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT6390.10788706807184367No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG6100.10299078485731555No Hit
ACGACCGTACGGGCCTTCACGGCTCAGCTCTGCTTCCTGGGAAC5990.10113357398283938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)