FastQCFastQC Report
Wed 31 May 2023
EGAF00007924624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592286
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTTTCCTGGGCGATCTGGTTGACCGTG164342.774673046467416No Hit
TACAGGAAGGTGTCCTGACCTGGTACGAAGA62141.0491553067268178No Hit
AAGATTCTGCTGCAGCGTTACGGTGATTTCC49520.8360825682187321No Hit
GCGCTGACGGCCACCTTCTCCAACATCGTGG49130.829497911481953No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47720.8056918448182129No Hit
ATGCACGTGATTTCTCGTAAACCGTTCAACG43610.7362996930536937No Hit
CGTACCGTAAAAGGTGGCACCATCGAGCCGC37400.631451697321902No Hit
ATGCTGTGGGCTCGTCAGGTCATTTTCATGA36230.6116977271115643No Hit
GTTGGCGGCGTAGGCGCAGGCGCAGACCCGT29240.4936804179062142No Hit
TTCTCCAGTCAGTCTGGTAGCTGGTACCTGA27620.46632876684574687No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA24860.4197296576316172No Hit
TCTACTCTGATCCGCCTGATGTCGGTGTTGA18340.3096477039808471No Hit
ATGGACATCTGTCTGCGTTTCTTCTGCGCGG15010.2534248656898863No Hit
CAGTCCTTTACCGAATGGCAGAACCCGGAGG14480.2444764860219556No Hit
GACCAGAGCACTCTGGGCTCTAATTGGGGTG13610.22978763637837127No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12000.20260482267012897No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10690.18048712952863988No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10310.1740713101440858No Hit
CCGGCGTCTAGCGGTTTCGCAAGCGTTATGC9010.15212245435482183No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG8920.15060291818479588No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG8320.1404726770512894No Hit
GACAGCATGCGTACCATCGATCAGACCTACA7800.13169313473558383No Hit
ACTATTCTGACCTACGCACCGAAAGCAATTG7700.13000476121333274No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG6900.11649777303532415No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT6890.11632893568309904No Hit
GGTTCTTCCAAAGGTGACGTTGCAGAGGGTG6700.11312102599082201No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6590.11126381511634582No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6540.11041962835522028No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG6320.10670520660626792No Hit
AACAAATCTCGCGCGGCTGAACCGACCGTCG6070.10248427280064024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)