FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572282
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA66011.1534523189616308No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47710.8336798990707378No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC37560.6563197863990131No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA35390.6184014174829892No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA30890.5397688552147368No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27040.4724943297185653No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21160.3697477816880489No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17500.3057932977098703No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17410.30422064646450525No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17020.29740582440125674No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15680.27399079474804383No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15060.2631569750577512No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14060.24568307233147293No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13560.23694612096833378No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC12290.21475426450596036No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12170.21265739617880694No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11640.20339622773387944No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT10950.1913392348527474No Hit
CAGCGTCCGCGCGGCGCTCCGACCCCGACCC10430.1822528054350827No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10320.1803306761351921No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10240.17893276391708984No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG10160.17753485169898756No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG9890.17281689796289243No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9820.17159372477205295No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9770.17072002963573904No Hit
ATGTCTGACGAAGGCCCGGGTACCGGCCCGG9740.17019581255395066No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9590.16757472714500893No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9090.15883777578186978No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8870.15499351718208856No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8410.14695552192800054No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8350.14590708776442385No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA7750.13542274612865685No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7630.13332587780150346No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG7230.12633631671099213No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7190.125637360601941No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7120.12441418741110152No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7060.12336575324752481No Hit
CAGTCCATTGTAAACCGTACTGACCTGGATC6960.121618362974897No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6880.12022045075679472No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6860.11987097270226917No Hit
CCGCGTGTCCTGAGCAACTCCGGTTGGGGTC6600.1153277579934368No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG6250.10921189203923941No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6090.10641606760303486No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5930.10362024316683036No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5760.10064967970336303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)