FastQCFastQC Report
Sun 21 May 2023
EGAF00007924679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386467
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG29460.762290182602913No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT25010.6471445168668993No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT24010.6212690863644243No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC22330.5777983631202664No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC21880.5661544193941527No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA15870.410643082074278No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA15000.3881314575371248No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14930.3863201774019515No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA14840.38399138865672877No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT13490.3490595574783875No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC12420.3213728468407393No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12400.32085533823068985No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG12190.3154214978251701No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG11620.3006725024387593No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT11400.29497990772821486No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT11390.2947211534231901No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG11150.2885110501025961No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG10920.2825597010870268No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10770.27867838651165555No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG10240.26496440834534385No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9610.2486628871287846No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG9560.24736911560366087No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG9280.24012399506296786No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8960.23184385730217585No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG8920.23080884008207686No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG8870.22951506855695308No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC8680.2245987367614829No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG8620.2230462109313344No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT8430.21812987913586412No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG8320.21528358178059184No Hit
GCTCGGCTCCTGCTGCATCTGGGTGATTTCGCGGCCAGCACGCA8290.2145073188655176No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG7100.1837155565675724No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC6990.18086925921230015No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6910.17879922477210214No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6750.17465915589170616No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6400.16560275521583992No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6350.16430898369071614No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA6230.16120393203041916No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6190.16016891481032014No Hit
AGGAGCCCAGGACGGACGCGGAGTGGTCGGCTGGGTCGCAGACT6110.15809888037012215No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6110.15809888037012215No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6070.15706386315002316No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5710.14774870816913216No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA5320.13765729027316692No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG5200.1345522386128699No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5070.13118843264754818No Hit
GGCGCGCGGTGGGGTCTGCATAGTCGCCGGTGCCCACTGACGCA4960.12834213529227592No Hit
ACCCAGGGTGATCTGCTGAACACGATCGGAGTAGCCGCACGCTT4950.1280833809872512No Hit
ACCCAGCGTGATAGAACGCACGCGGTCAGAGTAACCACATTCCT4890.1265308551571027No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT4830.12497832932695419No Hit
CAGACGGTCGGTTGGATCCGGATACGCGCCGCGCAGCGGTGCTT4800.12420206641187993No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT4730.12239078627670667No Hit
TGGTGCCCAGGAAGGGCGCGGGGTAGTCGGCTGGGTAGCAGACT4610.11928573461640968No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG4540.11747445448123645No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC4500.11643943726113742No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG4350.11255812268576619No Hit
CCACTGTTCCGGAACCAGTGGACCTTCCATCGGCTGCTGAGGCA4320.11178185977069194No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC4270.11048808824556818No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG4250.10997057963551869No Hit
AATCGGCTGGGCTTTACCGGCGTCCATAACAGACAGAGCACATA4210.10893556241541968No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4040.10453673922999893No Hit
CTGGCACAGAGAATCACGGTTGGAGCGTTTGGTCGCTTCGTCCA4030.10427798492497418No Hit
TGGAGCCCAAGACGGACGCGGGGTGGTCGGCTGGGTTGCAGACT3970.1027254590948257No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG3920.10143168756970193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)