FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007924680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386467
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65911.705449624418126No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC28530.7382260322356113No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA27760.7183019507487055No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG27500.7115743388180621No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA24670.6383468704960579No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG23990.620751577754375No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC21740.5625318591238062No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17100.44246986159232227No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16550.42823837481596094No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16160.4181469569199957No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC13730.35526966079898153No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13680.3539758892738578No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA13440.3477657859532638No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12930.33456931639700155No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12630.32680668724625905No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT11970.3097289031146256No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC11900.3079176229794523No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC11880.30740011436940284No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11430.2957561706432891No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11240.29083983884781883No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA11210.29006357593274457No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA11170.2890285587126456No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10350.2678107057006161No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10060.2603068308548983No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9850.2548729904493786No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC9800.25357921892425483No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9560.24736911560366087No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA9400.24322904672326484No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA9250.23934773214789362No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9240.23908897784286884No Hit
ATCGTCACCCCACCGTCCGCGCGTCCGCGTA9090.2352076632674976No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC8570.22175243940621062No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8310.21502482747556714No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT8200.21217853012029486No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8100.2095909870700474No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT7740.20027583208915636No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7290.18863188836304262No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7050.18242178504244863No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6720.1738828929766319No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6560.1697428240962359No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6250.16172144064046867No Hit
CCGCGTATCATGGCACCGCCGGTAGTTCGTA6080.15732261745504791No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA5970.15447632009977566No Hit
ATGGCAGAACTGCCGCAGAGCCGTATCAACG5430.14050358762843917No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5410.13998607901838966No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG5360.13869230749326592No Hit
CCGCGTATTATGGCGCCGCCGGTTGTACGTA5140.1329997127827214No Hit
ATTGAAGTTATCGACGTTGAAACCACTGAAG5130.13274095847769668No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5070.13118843264754818No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5040.13041216973247394No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT5030.13015341542744915No Hit
CCGGCTGCCTCCCCGCCGCGTACCCCTGCCT4980.12885964390232543No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG4970.12860088959730068No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4940.1278246266822264No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC4920.12730711807217693No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4830.12497832932695419No Hit
GTAGGTCCAACCCCTCACCAGACCCCGCAGG4730.12239078627670667No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4660.12057950614153344No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA4630.11980324322645919No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4460.11540442004103843No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG4350.11255812268576619No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4280.11074684255059294No Hit
CCGCGCATTATGGCCCCTCCGGTTGTACGCA4260.11022933394054343No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4210.10893556241541968No Hit
CTGGCTGAAAACGGTCACAACGTATGGGCAC4200.10867680811039494No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC4200.10867680811039494No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4190.10841805380537017No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4160.10764179089029592No Hit
CAGTGCTTCTGGGAAATGCGTGCAGGCCGCG4090.10583051075512269No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4090.10583051075512269No Hit
ATCGTCACCCCGCCTTCCGCACGCCCGCGTA4030.10427798492497418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)