FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455922
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT77691.7040195472032498No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC32730.7178859541763722No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG26230.5753177078535364No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20860.4575344028145165No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18480.40533249108400127No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17460.3829602431994946No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17380.3812055570909059No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA17300.37945087098231717No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG16860.369800097385079No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG16180.35488526546207466No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG15830.3472085137369989No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15090.33097766723255295No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14170.31079877698378233No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11310.2480687486017345No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10610.23271524515158293No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10390.22788985835296388No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9990.21911642781002016No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9840.21582639135641624No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8960.19652484416193997No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8860.19433148652620405No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8690.19060277854545296No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7950.174371932041007No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7640.1675725233702256No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7340.1609924504630178No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7080.15528972061010438No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7050.15463171331938358No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6470.14191023903211514No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6410.1405942244506736No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6370.1397168813963792No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6300.13818153105136405No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6280.13774285952421686No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5890.1291887647448467No Hit
GTCCCGATCAACAACCAGTTCATTCCGTATC5800.12721474287268436No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5490.12041533420190295No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5230.11471260434898951No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5170.11339658976754795No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4950.1085712029689289No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4850.10637784533319296No Hit
GACGGTCCGTACGCACCGTTCCCGCCTCGCC4700.10308780887958906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)