FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007924687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426395
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA31820.7462564054456549No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA27150.6367335451869744No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT24160.5666107717022948No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC20640.48405820893772206No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA16860.3954080136962207No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT14410.3379495538174697No Hit
GAACTCGGTCAGGCCCATGCGGCGTTTGTTGGAGATGTCGTAAA13320.3123864022795764No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA13250.31074473199732644No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12450.29198278591446897No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12200.28611967776357605No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9790.22959931518896798No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT9630.2258469259723965No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9600.2251433529942893No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8740.2049742609552176No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8690.20380163932503897No Hit
CTGTTCGGTTTCCAGTTCGCCTACGCTCACTTTGGTGTTGTTAC8550.20051829876053892No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8480.1988766284782889No Hit
AAATTCGGTCAGGCCCATACGACGTTTGTTAGAGATGTCGTAGA8400.19700043387000316No Hit
GATGCTGGAAATGTTGGAGCGAGATTTAACCAGGGTAACAGAGC7900.18527421756821727No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7780.18245992565578864No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7590.17800396346111No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6970.16346345524689548No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT6940.16275988226878835No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6600.1547860551835739No Hit
GCGCTGTTTAGCCAGCAGTTCCGCTGCTTTACGTTCTTCCTGCT6530.1531443849013239No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6440.15103366596700243No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6420.150564617314931No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6290.14751580107646667No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6010.14094911994746653No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5670.13297529286225213No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT5670.13297529286225213No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5650.1325062442101807No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5490.1287538549936092No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5490.1287538549936092No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5420.12711218471135918No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC5390.12640861173325205No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG5370.1259395630811806No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5220.12242169819064483No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG4900.11491691975750185No Hit
GCCACGGCTCAGCTGAGAGCGGATTTTACGGTACTGGCCAGAAC4830.11327524947525182No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA4810.1128062008231804No Hit
GGCTGCCGGTTTAGCCGCCGGCTTGGCCGCTGCGGACTTAGCAA4610.10811571430246601No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG4580.10741214132435886No Hit
AATATCAACGGTCTGGACACCCAGGCCCGGTGCCACTTTCTTGA4270.1001418872172516No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)