FastQCFastQC Report
Wed 31 May 2023
EGAF00007924692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435612
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT73211.6806240415782852No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29000.6657300533502291No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG25760.5913519370448932No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18950.43502015555127044No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15800.3627080980321938No Hit
GTCCACATCAAACTGCCGAAGCTGGCTGCAA14370.3298807195394066No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14220.3264372882289744No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14160.3250599157048015No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14020.3218460464817315No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12670.29085516468784145No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10870.24953398896265483No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10640.2442540609533254No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG10460.24012194338080675No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10240.23507157745883953No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10150.2330055186725802No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9710.22290478682864567No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9490.2178544209066784No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9030.20729456488801962No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8650.19857120556825797No Hit
AGCGTGCACATCAAGATCCCGAAAGTTTCCC8490.19489821217046363No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG8470.19443908799573933No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7560.17354893804578386No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA7400.1698759446479895No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7080.16252995785240076No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG6990.16046389906614142No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6320.14508323921287752No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT6310.1448536771255154No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6300.1446241150381532No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6260.1437058666887046No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT6180.14186936998980743No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6070.1393441870288238No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6060.13911462494146165No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6050.13888506285409952No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5860.13452338319421872No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5820.13360513484477013No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5070.11638797829260901No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5070.11638797829260901No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA5020.11524016785579827No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4700.10789418106020954No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4670.10720549479812311No Hit
GTGCGTGCATCTAACCTGTCCGCCGAACAGC4650.1067463706233988No Hit
GTTCTGGTTAACGAAGGTAACGGTTATGTGG4460.102384690963518No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4450.10215512887615584No Hit
GCGAAAACTGCGGCGGCCAAACCGGCTGCCA4450.10215512887615584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)