FastQCFastQC Report
Wed 31 May 2023
EGAF00007924694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409064
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT67241.6437525668355075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57571.4073592396299845No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26290.6426867189486242No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG23610.5771712983787378No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17550.42902822052295No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15100.3691353920168971No Hit
GTCCACATCAAACTGCCGAAGCTGGCTGCAA13490.3297772475700624No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13190.3224434318346273No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13030.3185320634423953No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12110.2960416951870612No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10130.24763851133318993No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10050.2456828271370739No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9800.2395713140242114No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG9730.23786009035260988No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9650.23590440615649383No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9030.22074785363659474No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8340.20388007744509418No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG8170.19972424852834764No Hit
AGCGTGCACATCAAGATCCCGAAAGTTTCCC7840.1916570512193691No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7670.18750122230262256No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7230.17674495922398453No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA7030.17185574873369447No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6760.16525531457180295No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6550.1601216435569984No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG6420.1569436567383099No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT6000.14667631470870085No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5950.14545401208612832No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT5690.1390980384487513No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5570.13616451215457728No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5570.13616451215457728No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5570.13616451215457728No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5510.13469774900749026No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5490.13420882795846126No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5290.12931961746817125No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA5210.12736393327205522No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5120.1251637885514247No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4880.11929673596307669No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4790.11709659124244616No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG4730.11562982809535916No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4550.11122953865409814No Hit
GCGAAAACTGCGGCGGCCAAACCGGCTGCCA4520.11049615708055463No Hit
GTTCTGGTTAACGAAGGTAACGGTTATGTGG4430.10829601235992413No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4230.1034068018696341No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)