FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464261
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG31240.6728973573054812No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT30310.652865521764697No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG25640.5522755518986088No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22790.49088766878975404No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA22450.48356420203290823No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA21200.4566396918974456No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG20520.44199275838375396No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT17570.37845091446406226No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG16610.35777289068002693No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG14950.32201714122013264No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT14930.32158634905796524No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG14890.32072476473363043No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14660.3157706548687053No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT14660.3157706548687053No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13400.28863074865215904No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12990.2797995093277273No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG12570.27075287392221187No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12480.2688143091924586No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG12400.26709114054378896No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG12250.26386019932753346No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG12240.26364480324644973No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT12030.259121485543692No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG11890.2561059404085202No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC11690.2517980187868462No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10630.22896603419197392No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA10630.22896603419197392No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG10460.225304300813551No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG10300.2218579635162118No Hit
TTCGTCGGTCTCTTCCAGACGACGACCCGGAGACATACGGGCAG10300.2218579635162118No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG9820.21151895162419415No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG9730.20958038689444083No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT9630.20742642608360382No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG9590.20656484175926904No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG9590.20656484175926904No Hit
CATACGGTCCAGGTCTTCGGTCGCGGAGCGAGCACGGGTGCTGG8970.19321028473207957No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8900.1917025121644937No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC8830.19019473959690777No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG8720.18782538270498708No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8420.18136350027247602No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG8170.17597859824538353No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7970.17167067662370952No Hit
CTTTGGGTGTTTCTGTTTCTGACGCGCTTCGCTAGAGGTACGTT7660.1649933981101148No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT7600.16370102162361258No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG7470.16090087256952446No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC7260.15637755486676677No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG7210.15530057446134826No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7130.15357740581267865No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC7110.15314661365051124No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6880.14819250378558613No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG6770.14582314689366543No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6620.1425922056774099No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG6570.1415152252719914No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG6480.1395766605422381No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6460.1391458683800707No Hit
TACCTGGATGTCCAGATCGAAGTAGAAACGGCGTGCCATGGCCG6240.1344071545962293No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5970.12859146040696937No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT5960.12837606432588564No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG5800.12492972702854645No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5680.12234497405554202No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5640.12148338973120723No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG5450.11739086419061692No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG5430.1169600720284495No Hit
GGTATCGGAAGAGTCGTCATCCTCTTCTTCGGAGTCTTCTACCG5290.1139445268932777No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG5250.11308294256894291No Hit
TTCTGCGGAGTTCGGTACGATTTCCAGCTGCGGAACTTTATATT5170.1113597739202733No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG5150.1109289817581059No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG5100.10985200135268738No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG5040.10855962486618519No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT5040.10855962486618519No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG4880.10511328756884597No Hit
AACCGTGGATTCCGCCTTCAGGCGGCCACGGGTCATAACACCCG4870.10489789148776228No Hit
TTTGGAGGTGAACGGTTTACCTTTATCCGGCAGGTCTGCCATCG4830.10403630716342747No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA4780.10295932675800896No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4740.10209774243367417No Hit
ACCGTGGTCGCCGTTGTCGCCATCGTCACCATCGTCGCCGTCAT4710.10145155419042307No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT4650.10015917770392085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)