FastQCFastQC Report
Wed 8 May 2024
EGAF00007924708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433139
Total Bases13.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT59001.3621493331240087No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC35480.819136582021014No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA22710.5243120568685803No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG21100.4871415411680777No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20970.48414019517983836No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG18510.427345494171617No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG15800.364778973955243No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15300.3532353355389378No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14880.34353867926924153No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG14400.33245678638958853No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14280.3296863131696753No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG14120.32599234887645767No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC12860.29690238006736863No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10790.2491117170238653No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10480.24195466120575612No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10380.23964593352249508No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10320.23826069691253848No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9520.21979087544645023No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9420.2174821477631892No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9300.21471167454327594No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG9130.2107868374817322No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9080.20963247364010168No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8690.20062843567538366No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8610.19878145352877483No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8480.19578010754053551No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7820.18054250483101267No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7050.16276530166990272No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6550.15122166325359757No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6290.14521897127711889No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6220.14360286189883617No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5990.13829278822733582No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5840.13482969670244424No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5780.13344446009248762No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5590.12905787749429165No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5230.12074645783455196No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5040.11635987523635599No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG4530.10458536405172474No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT4370.1008913997585071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)