FastQCFastQC Report
Wed 31 May 2023
EGAF00007924717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376123
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT37270.9908992537015817No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC36620.973617672942096No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA36370.9669709111115248No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA34680.9220388011368621No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT29480.7837861550609775No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA29380.7811274503287489No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT27430.729282708050292No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA27310.7260922623716178No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA23480.6242638711272642No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT20920.5562010299822132No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA19440.5168521999452307No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT19080.5072808629092078No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT18400.4892016707300538No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT13590.36131797310986036No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT13560.36052036169019175No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT13480.35839339790440894No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG13240.3520125065470604No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13170.35015141323450044No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC12990.345365744716489No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC12240.3254254592247749No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG11910.3166517336084207No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT11600.30840974893851214No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA11130.29591383669703797No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT11120.29564796622381506No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG11110.29538209575059227No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC10530.2799616083036666No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT10280.27331484647309523No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC9980.2653387322764096No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG9200.2446008353650269No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG8510.22625577271264985No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT8500.22598990223942703No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT8410.22359706798042128No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA8070.21455747189084423No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT8040.21375986047117565No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG7830.2081765805334957No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC7760.2063154872209357No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT7740.20578374627449No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC7710.20498613485482142No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7660.20365678248870714No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG7660.20365678248870714No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA7600.20206155964937003No Hit
CACGCTAGATACCATCTGGCAGAACATGGAGATGTCCTGGCCAT7490.19913698444391864No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC7180.1908949997740101No Hit
CAGTTCGCGGTTAGCCAGAACTTTCGCTTCTGCCAGTTCAATGG7060.18770455409533585No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7030.18690694267566726No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6960.18504584936310728No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG6740.17919669895220447No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6500.17281580759485593No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC6270.16670078671073027No Hit
CGGGTTCTGCATGATATCATCCATGATCACTACGCTCTGCTGGT6200.16483969339817028No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6190.16457382292494743No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6090.16191511819271887No Hit
CGGTTCGCGCGGCTCACGTGGTACGCGCGGTTCACGCGGAACAC5850.15553422683537035No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT5830.15500248588892462No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5770.1534072630495875No Hit
CAGACGAGAACGTTTGGACAGAGAACGAGTGTAGGAAACGTTGC5650.15021681737091325No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA5580.14835572405835323No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT5570.14808985358513038No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA5550.14755811263868468No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5530.14702637169223898No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT5150.13692329370977047No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT5120.1361256822901019No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5100.13559394134365618No Hit
ATCGTCGGACGGACGTTTGTTGTCGCTGCTAGTACGAGATGGAA4990.1326693661382048No Hit
CTGGATCGGGGTCGGCTGAGATTCCTTCAGCAGAGCCAGCAGAG4890.13001066140597622No Hit
TTCGCGACCCTGTTCGCCCGGGTGGGTGTGGTTGTAACCAACGC4890.13001066140597622No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG4840.12868130903986194No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG4660.12389564052185056No Hit
GCCCTGACCGCGTGGGCCGGTGGACGGACCCTCACCCGGGTCGT4610.1225662881557363No Hit
GCGGAGCAGAGACAGGACGTTCTGCGGAACCTGGTTAGCCTGGG4610.1225662881557363No Hit
CTGTTTTTTACGCGCGCCACGTGGTGCGGTATCGTGCGGCTGAC4520.12017345389673059No Hit
CTGTTTTTTACGAGCACCGCGCGGTGCGGTATCGTGTGGCTGAC4450.1183123605841706No Hit
ACGCAGCAGAGACAGAACCGTCTGCGGGACCTGGTTTGCCTGCG4350.11565365585194205No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG4320.1148560444322735No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC4160.1106021168607078No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG4140.1100703759142621No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG4110.10927276449459351No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG3950.10501883692302784No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG3870.102891873137245No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT3860.10262600266402215No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG3850.1023601321907993No Hit
GATACGCGGTACACGAGGTTCGCGCGGGGAACGTGGCACACGCG3820.10156252077113072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)