FastQCFastQC Report
Sun 21 May 2023
EGAF00007924718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376123
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT87172.3175929150836296No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61021.6223416276058629No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC51031.3567370248562305No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG37631.0004705907376046No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG32140.8545077009382569No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA29560.7859131188467603No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT29020.771556113292726No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC27980.743905584077549No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA26550.7058861064066808No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT26390.7016321788351151No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG22520.5987403056978701No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC22010.5851809115635045No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG21190.5633795327592304No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA18690.4969119144535165No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC14450.3841828338070259No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC14050.3735480148781117No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC13820.367432993993986No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG13680.363710807368866No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT13660.3631790664224203No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC13010.34589748566293477No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT12080.32117153165320916No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT12020.31957630881387206No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11640.30947323083140355No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG11520.3062827851527293No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG11200.2977749300095979No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC11140.29617970717026076No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT11020.2929892614915865No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG10270.2730489759998724No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9860.2621482865977353No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8580.22811686602520984No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8560.22758512507876413No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8530.22678751365909555No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA8410.22359706798042128No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8380.22279945656075276No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC8350.22200184514108418No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT8320.2212042337214156No Hit
GGCAAATCCCTGACCACCTCTATTGTTGGTC8300.2206724927749699No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8230.2188113994624099No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC8170.2172161766230728No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8090.21508921283728993No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7960.21163289668539284No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7910.21030354431927856No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7750.20604961674771283No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7240.19249022261334722No Hit
ATCAAAGACCTGCAGGCACAGGTGGTAGAAA6940.18451410841666158No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6930.18424823794343873No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6760.17972843989865017No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG6670.17733560563964448No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6610.17574038280030735No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG6380.16962536191618166No Hit
ATCCACGGTTCTCCGGGCACTGGTAAATCCC6230.16563730481783884No Hit
CCTACCGATGGCCCGCCGCACCGTGGTGGCG6010.15978815440693603No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5940.15792706109437604No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT5850.15553422683537035No Hit
GGCGGTACCTACCGTAGCTATGAACAGCTGA5830.15500248588892462No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5660.1504826878441361No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA5580.14835572405835323No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA5500.1462287602725704No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5500.1462287602725704No Hit
ATCCACGTTCAGTGCAACTCTTCCAAATTCC5240.13931612796877615No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT5200.13825264607588475No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5000.13293523661142764No Hit
GGTCGTTCTGGCTATACTATCCATGTACAGT4830.1284154385666391No Hit
AACGCTAAAATCGCGGAGGCAGAAGCGCTGC4730.12575673383441055No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4520.12017345389673059No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG4490.11937584247706202No Hit
GCCCAGTCCGCGACCCCGTCTGTTTCTTCCT4380.11645126727161062No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4360.1159195263251649No Hit
CTGGCCTCTTCTGCACCGTCCACCGCGGTTG4350.11565365585194205No Hit
CGTTTCAACAGCGCTATCACCAACCTGGGCA4310.11459017395905062No Hit
CTGCGTGTCTGCGCTGCGCTGTCCATCCCGA4170.11086798733393065No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT4060.10794341212847926No Hit
CGCTTCGAATCCACCATCACCAACCTGAACA4050.1076775416552564No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC4050.1076775416552564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)