FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007924719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390868
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC38510.9852430999723691No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT38150.9760328294974262No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA37250.9530071533100688No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA36530.9345866123601829No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT31340.8018052130130888No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA31300.8007818496269841No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT29650.7585681099501622No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA28450.727867208367019No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA24650.6306476866870657No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT21560.5515928651104721No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT19790.5063090352753359No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA19660.5029831042704954No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT19110.48891185771155476No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT14340.36687577391856074No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT14040.35920054852277494No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT13950.35689798090403924No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC13830.3538278907457249No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13420.343338416038151No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG13230.3384774399541533No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC13130.33591903148889135No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG12930.3308022145583675No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG12000.3070090158314316No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA11530.2949844960447005No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT11370.2908910425002814No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT11370.2908910425002814No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC11000.2814249311788123No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT10910.2791223635600765No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC9720.2486773028234596No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG9470.24228128166030477No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9000.2302567618735737No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA8680.22206985478473554No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA8350.21362710684937114No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG8340.21337126600284498No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT8310.21260374346326635No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG8210.21004533499800446No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT8130.20799860822579488No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT8030.20544019976053296No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG7900.20211426875569247No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC7810.19981170113695673No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC7810.19981170113695673No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT7650.19571824759253764No Hit
CAGTTCGCGGTTAGCCAGAACTTTCGCTTCTGCCAGTTCAATGG7570.1936715208203281No Hit
CACGCTAGATACCATCTGGCAGAACATGGAGATGTCCTGGCCAT7510.19213647574117093No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC7390.1890663855828566No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7360.18829886304327803No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7340.18778718135022565No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6770.17320425309823265No Hit
CGGTTCGCGCGGCTCACGTGGTACGCGCGGTTCACGCGGAACAC6750.17269257140518027No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG6640.16987832209339215No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6320.16169141500455397No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC6280.1606680516184492No Hit
CGGGTTCTGCATGATATCATCCATGATCACTACGCTCTGCTGGT6270.160412210771923No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6200.15862132484623964No Hit
CAGACGAGAACGTTTGGACAGAGAACGAGTGTAGGAAACGTTGC6120.15657459807403012No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA5800.14838769098519194No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT5690.1455734416734038No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT5630.14403839659424666No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT5560.1422475106685633No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5560.1422475106685633No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5470.13994494304982755No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT5400.13815405712414422No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5280.13508396696582992No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5240.13406060357972513No Hit
GCCCTGACCGCGTGGGCCGGTGGACGGACCCTCACCCGGGTCGT5190.13278139934709415No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA5130.13124635426793702No Hit
ATCGTCGGACGGACGTTTGTTGTCGCTGCTAGTACGAGATGGAA5000.1279204232630965No Hit
CTGGATCGGGGTCGGCTGAGATTCCTTCAGCAGAGCCAGCAGAG4990.1276645824165703No Hit
TTCGCGACCCTGTTCGCCCGGGTGGGTGTGGTTGTAACCAACGC4940.12638537818393933No Hit
GCGGAGCAGAGACAGGACGTTCTGCGGAACCTGGTTAGCCTGGG4910.12561785564436076No Hit
CTGTTTTTTACGCGCGCCACGTGGTGCGGTATCGTGCGGCTGAC4880.12485033310478218No Hit
CTGTTTTTTACGAGCACCGCGCGGTGCGGTATCGTGTGGCTGAC4590.11743094855552257No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG4410.11282581331805111No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4360.11154660908542013No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG4260.10898820062015821No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG4260.10898820062015821No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG4130.10566226961531772No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC4130.10566226961531772No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG4130.10566226961531772No Hit
ACGCAGCAGAGACAGAACCGTCTGCGGGACCTGGTTTGCCTGCG4070.10412722453616054No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG3940.10080129353132003No Hit
CGGTTCACCATCCGGACCACCTTCCTGGTGATATTCAAAGTAGT3930.10054545268479384No Hit
CGGGCCTTCACCTGGATCGTCCGCCGGACCCTGTTCGATAGCAC3910.10003377099174146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)