FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390868
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT89672.2941248708003728No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC54341.3902391600233326No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54191.3864015473254399No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG38590.9872898267445788No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG33150.8481124062343297No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA32180.823295844121289No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC30640.7838963537562552No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT30260.77417440158826No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT28340.7250529590552309No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA27170.6951195800116663No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG23160.5925274005546629No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC23150.5922715597081367No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG21450.5487786157986839No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA19680.5034947859635478No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC15430.3947624261899158No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC14450.36969002323034883No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC13980.3576655034436178No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT13960.3571538217505654No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG13800.3530603682061463No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC13450.3441059385777296No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT12610.3226153074695294No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT12410.31749849053900553No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG12150.31084662852932443No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11760.300868835514803No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG11720.29984547212869817No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC11580.29626370027733145No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT11350.29037936080722904No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG10180.26044598176366446No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9980.2553291648331406No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA9360.23946703234851663No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC9090.23255932949230942No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT9080.23230348864578323No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8840.22616330832915457No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC8820.22565162663610222No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8750.22386074071041887No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC8710.2228373773243141No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8520.21797640124031642No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8430.2156738336215807No Hit
GGCAAATCCCTGACCACCTCTATTGTTGGTC8400.21490631108200212No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT8310.21260374346326635No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8080.2067194039931639No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7740.19802081521127338No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7700.19699745182516862No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA7640.19546240674601145No Hit
ATCAAAGACCTGCAGGCACAGGTGGTAGAAA7450.1906014306620138No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA7100.18164700103359702No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6980.1785769108752827No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6940.17755354748917793No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG6910.17678602494959936No Hit
CCTACCGATGGCCCGCCGCACCGTGGTGGCG6680.1709016854794969No Hit
ATCCACGGTTCTCCGGGCACTGGTAAATCCC6340.16220309669760635No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG6180.15810964315318726No Hit
GGCGGTACCTACCGTAGCTATGAACAGCTGA6100.15606291638097772No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT6090.15580707553445153No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5820.14889937267824432No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5780.14787600929213954No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5650.14455007828729904No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA5620.14378255574772045No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA5560.1422475106685633No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT5410.1384098979706704No Hit
ATCCACGTTCAGTGCAACTCTTCCAAATTCC5320.13610733035193467No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5300.13559564865888227No Hit
AACGCTAAAATCGCGGAGGCAGAAGCGCTGC4980.1274087415700441No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4910.12561785564436076No Hit
GGTCGTTCTGGCTATACTATCCATGTACAGT4820.12331528802562503No Hit
CGTTTCAACAGCGCTATCACCAACCTGGGCA4760.12178024294646787No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4670.11947767532773212No Hit
GCCCAGTCCGCGACCCCGTCTGTTTCTTCCT4620.11819847109510116No Hit
GGTTCTCGTGAGCGTGCACGCGGCCGTGGCC4420.11308165416457729No Hit
CTGGCCTCTTCTGCACCGTCCACCGCGGTTG4420.11308165416457729No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC4230.10822067808057964No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG4030.10310386115005578No Hit
CTGCGTGTCTGCGCTGCGCTGTCCATCCCGA4010.10259217945700339No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG3980.10182465691742482No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC3920.10028961183826765No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT3910.10003377099174146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)