FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007924753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533618
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT38090.7138065057775412No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC37610.7048113069649075No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA29150.5462709278922375No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT23360.43776634221484284No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA23220.435142742561158No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA18830.35287415342061174No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA18290.34275455475639877No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT17890.33525855574587066No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG16910.3168933581700767No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT15950.2989029605448092No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT12940.24249556799058503No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG12180.22825316987058158No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT11980.22450517036531753No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11950.22394297043952788No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG11370.2130737718742621No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG9370.17559377682162144No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA9170.1718457773163574No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA9160.17165837734109418No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG8380.15704117927056435No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG8120.15216877991372105No Hit
CAGTTCAACAGTACGCGGGATGGACATCAGGCTAGCCCAGCCTT7840.14692158060635135No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA7710.1444853809279297No Hit
GTAGCCAGAACCATCGGAACCGGTAACCGGAGCTGCTTTGGAGC7450.13961298157108643No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7140.13380358233792713No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6770.12686978325318862No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT6570.12312178374792454No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG6530.12237218384687173No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC6350.11899898429213408No Hit
GTCGCCCGGCAGCGGTGCAGTCGGAGCCCACGGGGAGTTACCGT6130.1148761848363436No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6120.11468878486108039No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5850.1096289855289739No Hit
GTCGAAAGCGACGGTTTTCATGCCGGTGGTGGATTTCACCGGGC5520.10344478634528821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)