FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541484
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT39050.7211662763812043No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC38550.7119323932009072No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA30570.5645596176433653No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA23900.4413796160182018No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT23070.42605136993890863No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT19450.35919805571355756No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA19280.3560585354322565No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA18790.3470093299155654No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG17620.3254020432736701No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT16330.3015786246685036No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT14040.2592874397027429No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT13030.24063499567854268No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG11720.21644222174616426No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG11610.21441076744649887No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11560.21348737912846916No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA9770.18043007734300553No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA9660.17839862304334014No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG9270.17119619416270843No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG8700.1606695673371697No Hit
CAGTTCAACAGTACGCGGGATGGACATCAGGCTAGCCCAGCCTT8070.14903487452999536No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA8040.1484808415391775No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG7970.14718809789393594No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG7180.13259856246906648No Hit
GTAGCCAGAACCATCGGAACCGGTAACCGGAGCTGCTTTGGAGC7030.12982839751497735No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6740.12447274527040504No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6730.12428806760679909No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT6690.12354935695237533No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6550.12096386966189213No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6330.1169009610625614No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC6280.11597757274453169No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA5680.10489691292817516No Hit
GTCGAAAGCGACGGTTTTCATGCCGGTGGTGGATTTCACCGGGC5560.10268078096490386No Hit
GTCGCCCGGCAGCGGTGCAGTCGGAGCCCACGGGGAGTTACCGT5470.10101868199245037No Hit
CTGACGGTTGATTTCATCTTTGGTGCGGACGTTGTCGGCGTGTG5460.10083400432884444No Hit
TTTCACGACCACTTTAACGGTTTCGTCACCAACAGTTACCAGTA5420.10009529367442066No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)