FastQCFastQC Report
Wed 31 May 2023
EGAF00007924789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514588
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22970.4463765186906807No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT22100.42946978942377206No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG18990.36903309055010997No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA18330.35620729593383443No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15010.2916896624095393No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14000.27206231004220854No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12660.24602206036674No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG12100.23513956796505164No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA11610.22561738711357437No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11510.22367408489898716No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11430.2221194431273174No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10680.20754467651791336No Hit
GCGCAGACCCGGGAAACGGTCACGTGCCGCCTGCAGTGCGCGAG10320.20054878854539943No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC9390.18247607794973844No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9370.18208741750682098No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG8970.1743142086484722No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7930.15410386561676526No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7770.15099458207342575No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7520.14613632653695774No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC7350.14283271277215948No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG7330.14244405232924204No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA7280.14147240122194843No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7190.13972342922881995No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT7060.13719713634985659No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7050.13700280612839788No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG6640.12903526704859034No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA6530.1268976346125444No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6490.12612031372670954No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG6380.1239826812906636No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5890.1144605004391863No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT5880.11426617021772759No Hit
TTCGTGCAGAGGATATGCCATGCCCTGGGCCGCCAGATGGATGG5500.10688162180229621No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5340.10377233825895668No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5180.10066305471561716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)