FastQCFastQC Report
Wed 31 May 2023
EGAF00007924840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473264
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT54751.1568595963352377No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC38940.8227965786537746No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG31040.6558707190912472No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23910.5052148483721559No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22170.46844889955711827No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG22140.4678150038878935No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22100.46696980966226037No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG16560.3499104094120829No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15170.32053991007133437No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC15030.31758173028161873No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG14180.2996213529869164No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11480.24257074275668547No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10480.22144088711585921No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT10400.21975049866459312No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10220.21594712464924443No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9430.19925453869299165No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9330.19714155312890902No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9320.19693025457250077No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8900.18805571520335373No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8280.17495520470604145No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7780.16439027688562832No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7310.15445924473443998No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7190.15192366205754082No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG7110.15023327360627473No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7050.14896548226782513No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6880.14537340680888466No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6840.14452821258325163No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6210.1312164035295311No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5890.12445484972446667No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5620.11874978870144359No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5400.11410122046046182No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC5370.11346732479123701No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5180.10945265221948004No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5020.10607187531694784No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4900.10353629264004868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)