FastQCFastQC Report
Sun 21 May 2023
EGAF00007924842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474756
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT56261.1850297837204795No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC38570.8124173259526999No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG32240.6790856776954899No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA24450.5150013901878018No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23170.48804017221477974No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22210.46781925873501334No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22140.4663448171271137No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17760.3740868993756793No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16290.34312362560978693No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC15080.3176368492446646No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG14960.3151092350596938No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11360.23928080951056965No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11070.23317240856355684No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10610.22348322085450212No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9920.20894943929092No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9800.20642182510594917No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9340.1967326373968944No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9220.1942050232119236No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9100.1916774090269528No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8180.1722990336088433No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7490.15776525204526115No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG7460.15713334849901844No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7360.1550270033448761No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6990.14723352627454947No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6710.1413357598429509No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6550.13796560759632318No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6420.13522735889593812No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6200.13059339955682497No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6200.13059339955682497No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC5670.11942977023987059No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5490.11563834896241437No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5200.1095299480154016No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA4990.10510662319170268No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4950.10426408513004574No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT4880.10278964352214612No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG4860.10236837449131765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)