FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007924888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510350
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA51601.01107083374155No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA27920.5470755363965906No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA25520.5000489859900069No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17660.34603703340844516No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16870.33055746056627805No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT14690.2878416772802978No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11430.22396394631135497No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG10390.203585774468502No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG10130.19849123150778875No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9990.19574801606740472No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG9520.1865386499461154No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG9460.18536298618595082No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA9050.1773292838248261No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG8000.15675516802194572No Hit
GAACTGGCTCGCCGTCAACAGGAAGAACGTA7570.14832957774076613No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7450.14597825022043695No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7180.1406877632996963No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6940.13598510825903792No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6900.1352013324189282No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6560.1285392377779955No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6120.11991770353678849No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6060.11874203977662388No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG5620.11012050553541688No Hit
GTGCGTGCATCTAACCTGTCCGCCGAACAGC5560.10894484177525227No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5400.10580973841481336No Hit
TCCATCTCTAGCAGCGAGGAAATCGTCCCAA5120.10032330753404527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)