FastQCFastQC Report
Sun 21 May 2023
EGAF00007924948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463816
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA110842.389740759266606No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA57811.2463994342584128No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA54761.1806405988581679No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC49521.067664763613157No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT41240.8891456957069184No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA26750.5767373268710005No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT23340.5032167928661365No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22420.4833813408765545No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19970.4305586698173413No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13510.29127930041223243No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13310.2869672456318885No Hit
GAATGCGACAACTATCGCCAGGAATACGATA13210.28481121824171657No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC12660.27295306759577076No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11320.24406230056746642No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9870.21279990340997293No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9420.2030977801541991No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9410.20288217741518186No Hit
ATCACTCTGTCTCAAAACGGCTACAACACTG9230.19900132811287233No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9100.1961984925056488No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8430.18175310899149663No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8410.18132190351346225No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8390.18089069803542784No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG8130.17528502682098074No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8050.17356020490884316No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7620.16428928713110372No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7510.16191765700191454No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7260.15652758852648463No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG7100.1530779447022095No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7090.1528623419631923No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6930.14941269813891717No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6890.14855028718284835No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA6650.14337582144643565No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6390.13777015023198855No Hit
CTGGGCTGGAAAGCTGGTATCCTGACCGGTT6340.13669213653690257No Hit
AACGTAACCACCAACCAGGACGGCACCGTCT6090.13130206806147265No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5830.12569639684702555No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA5600.12073753384963003No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5580.12030632837159565No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG5300.11426945167911412No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5040.10866378046466701No Hit
GTACTGCCGACCCCGATGCAGCTGGCACTGC5010.10801697224761544No Hit
CAGGTTAGCTATGGTGCTCGCCCGACCCAGA4990.10758576676958105No Hit
ATGGAATCTTTCGAAGGCCAGGGCGACTCCC4760.10262690377218552No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)