FastQCFastQC Report
Sun 21 May 2023
EGAF00007924959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497987
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT24680.4955952665431025No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC23660.47511280414950596No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC23070.4632651053139941No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19470.3909740615718884No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT19050.3825401064686428No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC17830.3580414749782625No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA17000.3413743732266103No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA16310.32751858984270676No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA16180.32490807992979737No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14360.28836094114906613No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA14220.28554962278131757No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT12880.2586412898328671No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12710.25522754610060105No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12550.2520146108231741No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG12460.2502073347296214No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11610.23313861606829092No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10990.22068849186826162No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10580.21245534521985515No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10450.20984483530694575No Hit
CATCGCCTCAGCGTAAGCGCTGGAGAAGGTTGCGTAGGACGGAG9490.19056722364238424No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7610.1528152341326179No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7550.15161038340358282No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7160.14377885366485468No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT6930.1391602592035535No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA6880.1381562169293576No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG6830.1371521746551617No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC6820.1369513662003225No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA6780.13614813238096576No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6460.1297222618261119No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6420.1289190280067552No Hit
GCGGCTCGGACCCGGCGGAGAGTGGGAAGAGCTGCAAGAGGTGA6320.12691094345836337No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6000.12048507290350953No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5730.11506324462285158No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5520.11084626707122877No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT5130.10301473733250065No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC5110.1026131204228223No Hit
TTCGCGGTCACGGCCGTTAGCGCCATTAACTTCGTAGTTAATCA4980.10000261050991291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)