FastQCFastQC Report
Sun 21 May 2023
EGAF00007924970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465636
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA49241.0574783736652664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39500.8483021072253862No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27590.5925229148948964No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25990.5581613105515897No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA21900.47032445944901163No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA18670.4009569706809611No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16630.35714592514324495No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14590.3133348796055288No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13140.282194675669407No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13070.28069135547938734No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG11680.25083971170613956No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11530.24761831129895456No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11490.2467592711903719No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10880.23365890953448618No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10100.2169076274171241No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9950.2136862270099391No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG9670.20767294624986038No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9330.20037110532690772No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9290.19951206521832504No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9020.19371354448539202No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7900.16966042144507726No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7260.15591577970775455No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG7090.15226485924627822No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6730.14453349826903417No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC6320.1357283371560618No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG6320.1357283371560618No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6280.13486929704747916No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6240.13401025693889648No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG6240.13401025693889648No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6090.13078885653171146No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6080.1305740965045658No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6030.12950029636883748No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6010.1290707763145461No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC5600.12026561520157375No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5500.11811801493011709No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5210.11188997414289274No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5100.10952761384429038No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4940.10609145340995971No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4930.10587669338281405No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG4900.10523241330137705No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4810.10329957305706602No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC4800.10308481302992037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)