FastQCFastQC Report
Wed 31 May 2023
EGAF00007924972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007924972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498455
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA54041.08415002357284No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG30210.606072764843366No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC28240.5665506414821799No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24760.49673491087460253No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA20780.4168881844900743No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17390.34887803312234805No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16240.32580674283536126No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14750.29591437542004795No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13550.2718399855553661No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13550.2718399855553661No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12140.24355257746436487No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12060.24194761814005278No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11790.23653088042049933No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11690.2345246812651092No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11310.22690112447462657No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11140.22349058591046334No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10390.20844409224503715No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10230.2052341735964129No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10020.20102115537009357No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG9740.19540379773500116No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8610.1727337472790924No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8420.1689219688838511No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7480.15006369682318363No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7210.1446469591036302No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG6990.14023332096177188No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6900.13842774172192074No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6760.13561906290437453No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6740.1352178230732965No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG6670.13381348366452336No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6550.1314060446780552No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6520.13080418493143814No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC6450.12939984552266504No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6290.1261899268740408No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG6270.12578868704296275No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC6010.12057256923894835No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5830.11696141075924608No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5630.11294901244846575No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5600.1123471527018487No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5340.10713103489783429No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5130.102918016671515No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC5000.1003099577695078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)