FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007925040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497776
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT50091.006275915271126No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC33900.6810292179614927No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA26420.5307608241458006No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC20050.4027916171129182No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG18900.37968885603162866No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT15750.3164073800263572No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14840.2981260647359455No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC14800.29732249043746584No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC13930.2798447494455337No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC13780.2768313458262351No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13700.2752241972292758No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT13430.26980007071453826No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC12390.24890713895406769No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC11980.2406705023946514No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC11630.23363922728295458No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG11490.23082671723827583No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11350.22801420719359713No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA10890.2187731027610813No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10800.21696506058950213No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA10640.21375076339558355No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10390.20872842403008582No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA9100.1828131529041175No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC9060.18200957860563788No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8950.17979974928481887No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC8360.16794702838224423No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8210.16493362476294557No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC8200.16473273118832568No Hit
CCTACCGATGGCCCGCCGCACCGTGGTGGCG8080.16232200829288676No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7560.15187554241265147No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7440.14946481951721255No Hit
GCGCGCGGCTCCACCTACGGTACCCCGCGTC7320.14705409662177366No Hit
CTGCGTGTCTGCGCTGCGCTGTCCATCCCGA7060.141830863681656No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6610.13279065282376007No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6380.12817010060750217No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5800.11651827327954743No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA5720.11491112468258816No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5690.11430844395872843No Hit
GCCAACTACATCGTCCGTCAGAGCCGCGGTG5420.10888431744399087No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5340.1072771688470316No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5060.10165214875767414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)