FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007925091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452724
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT18730.41371785016919804No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC18430.40709129624230217No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA18400.4064286408496126No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT17630.38942048577057986No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA16940.3741794117387194No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13650.30150820367376147No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12260.27080517047914404No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG12180.2690380894319718No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG11530.2546805559236974No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA9740.21514211749321882No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9520.2102826446134952No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8650.19106563822549721No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8120.1793587262879812No Hit
GGCTGCCGGTTTAGCCGCCGGCTTGGCCGCTGCGGACTTAGCAA7890.17427836827736104No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7510.16588473330329295No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT7120.15727021319832835No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT7020.15506136188936306No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6630.14644684178439846No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6180.13650701089405465No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC6030.1331937339306067No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5540.12237036251667682No Hit
ACGACCAGACAGCGGGGTGGTCTTGCCCGGTTTCGGGCGTTCTG5300.11706911937516012No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG5270.11640646398247056No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC5230.11552292345888444No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT4960.10955902492467817No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG4860.10735017361571289No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT4810.10624574796123025No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA4720.10425778178316147No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA4680.10337424125957537No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA4540.10028184942702396No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG4540.10028184942702396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)