FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007925331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530512
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA27810.5242105739361221No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT25390.4785942636547335No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA24410.4601215429622704No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT20700.39018910034080284No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC20070.37831377989564796No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG19730.3719048767982628No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG18230.3436303043097988No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG17940.33816388696202915No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15960.3008414512772567No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA15350.289343125131948No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG13780.25974907259402236No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG13070.24636577494948275No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG12880.242784329100944No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12220.23034351720601984No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG11690.2203531682600959No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11600.2186566939107881No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG11270.21243628796332598No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG11060.20847784781494105No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10430.19660252736978617No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG10130.19094761287209336No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT10100.19038212142232408No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA9940.1873661670235546No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG9840.18548119552432368No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9520.1794492867267847No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG9460.17831830382724614No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8870.16719697198178363No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8640.1628615375335525No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG8470.15965708598485992No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8160.15381367433724402No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG8010.15098621708839763No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7670.14457731399101245No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7470.14080737099255058No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7210.1359064450945502No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA7100.13383297644539613No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG7060.13307898784570377No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG6950.13100551919654974No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6830.12874355339747265No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6790.12798956479778026No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG6760.12742407334801098No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6720.1266700847483186No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG6700.12629309044847242No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT6280.11837621015170251No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC6190.11667973580239467No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6030.11366378140362518No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG5910.11140181560454807No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5800.10932834695539405No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG5440.10254244955816268No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5430.10235395240823959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)