FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007925332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530512
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT63861.203742799408873No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28310.5336354314322768No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC27210.5129007449407366No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25340.47765177790511804No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC21450.40432638658503484No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG21370.4028184093856501No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21260.4007449407364961No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG20080.3785022770455711No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG18610.35079319600687636No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC17080.3219531320686431No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA16270.30668486292487257No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16070.30291491992641073No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG15400.29028561088156346No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13390.25239768374702176No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG13390.25239768374702176No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13390.25239768374702176No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG13030.24561178634979042No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12760.24052236330186688No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC12380.23335947160478934No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11590.21846819676086499No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG11360.21413276231263384No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG11040.20810085351509483No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10710.20188044756763276No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT10060.18962813282263175No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9950.1875546641734777No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9070.1709669149802455No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8690.16380402328316795No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8670.16342702898332176No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC8490.1600340802847061No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG8470.15965708598485992No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8230.1551331543867057No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7970.15023222848870524No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7560.14250384534185845No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG7460.1406188738426275No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7350.13854540519347347No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7320.1379799137437042No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG7200.13571794794462708No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7140.13458696504508852No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG6990.13175950779624213No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6960.13119401634647285No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6840.12893205054739573No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG6800.12817806194770334No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6080.11460626715324065No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5910.11140181560454807No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5480.10329643815785505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)