FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007925628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419578
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48001.14400659710471No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA40010.9535771656283217No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA21220.5057462498033739No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA20640.4919228367550253No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC16440.39182225950836314No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16420.39134559009290293No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT15250.36346042928847555No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13730.32723355371349305No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG13180.314125144788335No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9830.23428301769873539No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9490.22617963763591037No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9140.2178379228653552No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8680.20687452630976838No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA8570.20425284452473677No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC8500.2025845015706257No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG7900.18828441910681684No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7560.18018103904399183No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7330.17469934076619842No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC7200.1716009895657065No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7020.16731096482656382No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6980.16635762599564324No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6720.1601609235946594No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6680.1592075847637388No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6630.15801591122508807No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6550.15610923356324688No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6360.15158087411637405No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6110.14562250642312036No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT5610.133705771036613No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG5130.12226570506556587No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5010.1194056885728041No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT4690.11177897792543938No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG4590.1093956308481379No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4570.1089189614326776No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC4420.10534394081672536No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4410.10510560610899522No Hit
GCACCGAAGCCTTCCAAACAGAGCAAGAAGA4370.10415226727807463No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT4250.10129225078531286No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA4230.10081558136985257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)