FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007925702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475033
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT85071.7908229533527145No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA65341.375483387469923No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA36940.7776301856923624No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA34600.7283704500529437No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC19270.405656028107521No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC18920.3982881189306848No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG18120.3814471836693451No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG17160.3612380613557374No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG16110.33913433382522895No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA14700.3094521854271177No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC14640.3081891152825172No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14480.30482092823024926No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG14060.2959794372180459No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT13790.29029562156734373No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG13730.28903255142274326No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT12430.2616660316230662No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG12190.2566137510446642No Hit
GTTCACGGCCACACCCAGGGTCCGTGGGACC12030.2532455639923963No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11380.2395623040925578No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11150.2347205352049226No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA11020.23198388322495492No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT10980.23114183646188788No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10580.22272136883121801No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10370.21830062332511635No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC10110.21282731936518093No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9800.20630145695141178No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC9430.19851252439304218No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG9420.1983020127022754No Hit
GGTCTGCAATCTTCCTCTTACCCAGGTTATG9290.19556536072230774No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC9040.19030256845313903No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8520.17935596053326822No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8420.17725084362560076No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT8390.1766193085533005No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA8320.17514572671793327No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7940.1671462824687969No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7760.16335707203499547No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7710.1623045135811617No Hit
GGTCTGCAGAGCTCTTCTTACCCGGGTTATG7650.16104144343656124No Hit
GGCCTGCAGTCTTCTTCTTATCCGGGTTACG7500.15788376807506005No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC7190.1513579056612909No Hit
GTACACGGCCACACCCAGGGCCCGTGGGACC7160.15072637058899066No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7130.15009483551669042No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG6750.14209539126755405No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6700.14104283281372032No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG6630.13956925097835307No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6620.13935873928758633No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6460.1359905522353184No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6420.1351485054722514No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC6300.13262236518305043No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC5910.12441240924314731No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG5800.12209678064471309No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC5730.12062319880934587No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA5630.11851808190167842No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5590.11767603513861143No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA5410.11388682470480997No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5400.11367631301404324No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC5280.11115017272484227No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5150.10841352074487456No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4880.10272970509417241No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT4850.10209817002187216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)