FastQCFastQC Report
Sun 21 May 2023
EGAF00007925703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476497
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA35020.7349469146710262No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA34230.718367586784387No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT29730.6239283773035297No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC28660.6014728319380814No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC26470.5555124166573977No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT18680.392027651800536No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG17400.3651649433259811No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG16760.3517335890887036No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC14550.3053534439881048No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT14420.30262520015865785No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT14400.30220547033874295No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG14200.29800817213959374No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG13890.29150235993091245No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG13230.27765127587372007No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG13170.27639208641397534No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC12750.26757776019576196No Hit
AACCAGCGGTACCAGGGACAGCTGCAGGCGTGGCGGACCGGTTT12300.2581338392476763No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT11450.24029532190129213No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT11320.23756707807184516No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA10610.22266666946486546No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG10040.21070436959729025No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG9930.20839585558775817No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT9910.20797612576784322No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG9870.20713666612801337No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG9800.20566761175831116No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG9760.20482815211848135No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC9130.19160666279116131No Hit
CCACTGCGGCGGCAGATGCGGAGCACGCGGGTCCCAAGGGCCCT9050.18992774351150166No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC9020.18929814878162926No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG8720.18300220148290547No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA8590.18027395765345847No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT8580.18006409274350102No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG8480.17796544364392639No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG8300.17418787526469212No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG8230.1727188208949899No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG8050.16894125251575562No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA7950.166842603416181No Hit
CCACTGAGGCGGCAGGTGCGGCGCGCGCGGGTCCCACGGGCCCT7950.166842603416181No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG7710.16180584557720196No Hit
CCATTGCGGCGGCAGGTGCGGAGCACGCGGATCCCACGGGCCCT7660.16075652102741464No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT7660.16075652102741464No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC7560.15865787192784003No Hit
CACCAGCGGAACCAGGGACAGCTGCAGACGCGGAGGACCGGTTT7250.15215205971915877No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT7080.14858435624988195No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6370.13368394764290226No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG6320.13263462309311497No Hit
CAGGTCATCTTCGTCCGCATCGCCGTCTTTGTTCGGCACCTGGG6070.12738800034417844No Hit
TGGGTCCGGCTTGCGGGTAGCTGCGCTAGTCGGGGCGACAGCCG6010.1261288108844337No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT6010.1261288108844337No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT5920.12424002669481654No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG5910.1240301617848591No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5850.12277097232511432No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG5820.12214137759524195No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG5780.1213019179554121No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT5730.1202525934056248No Hit
CGCCAAATTTAAATGGCCGCCTCATTTACGGCTGGCAGCGGAGG5620.11794407939609274No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT5450.1143763759268159No Hit
TTCCATCGGCTGCTGCGGGCTTACCTGGGTCAGCTGCGGTGGCT5240.10996921281770924No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA5140.10787056371813464No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG5100.1070311040783048No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG5090.10682123916834735No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA4910.10304367078911306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)