FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007925706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451293
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT80491.7835419561127694No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA60531.3412572319978373No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37480.830502578147678No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA33630.7451921478950482No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA30740.6811539288222951No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC18480.4094900652126224No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17000.3766954063103128No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16270.3605196623922817No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG16210.3591901491935395No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG15050.33348622735118866No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC13890.30778230550883795No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13720.3040153514457348No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA13450.29803254205139457No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG12840.284515824530848No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG12490.2767603308715181No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT12350.27365813340778605No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT11670.25859031715537356No Hit
GTTCACGGCCACACCCAGGGTCCGTGGGACC11470.2541586064928993No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG10970.24307932983671363No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT10600.2348806651111362No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10520.2331079808461465No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10450.23155688211428052No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10250.22712517145180625No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10100.22380138845495054No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC10100.22380138845495054No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10020.22202870418996085No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9390.20806881560316692No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC8810.19521685468199154No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG8570.1898988018870224No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA8420.1865750188901667No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC8390.18591026229079555No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8150.18059220949582644No Hit
GGTCTGCAATCTTCCTCTTACCCAGGTTATG7930.17571732776710475No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT7930.17571732776710475No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA7790.17261513030337275No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7700.17062086050525935No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7250.16064951151469223No Hit
GGTCTGCAGAGCTCTTCTTACCCGGGTTATG7220.1599847549153211No Hit
GGCCTGCAGTCTTCTTCTTATCCGGGTTACG6730.14912706379225912No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6560.145360109729156No Hit
GTACACGGCCACACCCAGGGCCCGTGGGACC6550.1451385241960323No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC6550.1451385241960323No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG6500.14403059653041372No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6500.14403059653041372No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6390.14159315566605288No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6290.13937730033481574No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC6070.13450241860609405No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5840.12940595134424865No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC5720.12674692494676407No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC5710.12652533941364036No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA5670.1256389972811455No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5670.1256389972811455No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5300.1174403325555681No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG5270.11677557595619696No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA5210.1154460627574547No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5160.11433813509183612No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5020.11123593762810413No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4890.10835532569749586No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC4840.1072473980318773No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC4790.10613947036625872No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG4580.10148617417066075No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT4530.10037824650504218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)