FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007925737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451323
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT31640.7010500240404323No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT26670.5909293344234617No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG25050.5550348641660185No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA19800.43871019203541584No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19690.4362729131907747No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT19090.42297866494727726No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG19080.42275709414321894No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA17180.38065864137214367No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG16300.3611604106150141No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG15650.3467583083512252No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG11820.2618966903968998No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11020.24417102607223654No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT10750.23818861436266267No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG10660.23619447712613803No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG9890.21913352521364965No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC9780.21669624636900842No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA9070.2009647192808698No Hit
CAGAGCGAAAGTAATGTTTTTGCCATCACGCTTAACCTTCAGGT8270.18323905495620652No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA8190.1814664885237402No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG7690.17038794832082566No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT6930.15354856721239554No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT6610.14645830148253025No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6260.13870332334049007No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG6180.13693075690802375No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG5390.11942666338741878No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5320.11787566775901073No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5280.11698938454277757No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT5020.111228543637262No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4950.10967754800885396No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG4950.10967754800885396No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT4870.10790498157638764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)