FastQCFastQC Report
Wed 31 May 2023
EGAF00007925741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449984
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT31910.7091363248471056No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT27110.6024658654529939No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG24500.5444638031574456No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20460.4546828331674015No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG19350.4300152894325132No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT19330.4295708291850377No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA19110.4246817664628076No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA17050.3789023609728346No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG16980.37734675010667046No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG16210.360235030578865No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG12190.2708985208362964No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11190.24867550846252312No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG10750.23889738301806288No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT10570.23489724079078367No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG10120.2248968852225857No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC9340.20756293557104255No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA8850.1966736595078936No Hit
CAGAGCGAAAGTAATGTTTTTGCCATCACGCTTAACCTTCAGGT7970.17711740861897313No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA7860.17467287725785807No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG7100.15778338785379034No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT6520.14489404067700185No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT6480.14400512018205092No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6420.14267173943962452No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG6060.13467145498506614No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5260.11689304508604752No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5250.11667081496230979No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG5050.1122262124875551No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT4980.11067060162139099No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4910.10911499075522686No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT4850.10778161001280046No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4670.10378146778552128No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT4630.10289254729057033No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA4540.10089247617693074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)