FastQCFastQC Report
Wed 31 May 2023
EGAF00007925742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449984
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT45651.0144805148627505No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39080.8684753235670601No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT30330.6740239652965438No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT27410.6091327691651258No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT24160.5369079789503627No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC21500.47779476603612575No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20750.4611275067557957No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG19840.44090456549566204No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA19640.43645996302090745No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG18950.4211260844830038No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC17960.39912530223296827No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC15180.33734532783387855No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC12690.282010027023183No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11660.2591203242781966No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT11080.24623097710140804No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC9310.2068962451998293No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT9140.20311833309628788No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG8300.18445100270231832No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT7290.16200576020480728No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7200.1600056890911677No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7040.15645000711136398No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT6760.15022756364670742No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6280.13956051770729627No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5510.12244879817949082No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5420.12044872706585123No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5360.11911534632342484No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5270.11711527520978522No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA5230.1162263547148343No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC4760.10578153889916087No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG4700.10444815815673444No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA4690.10422592803299674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)