FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007925749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458248
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA27130.5920374993453327No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA25020.5459925629789982No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC20710.45193868822122524No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT18470.4030568600408513No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC16900.3687959358251427No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT15700.3426092421570852No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA15020.327770115745186No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA14210.3100940975192472No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13800.3011469771826609No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC13650.2978736404741537No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12650.27605139575077253No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11260.24571847558527263No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA10930.23851713482655681No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10000.21822244723381226No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8770.19138108622405334No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8750.19094464132958575No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC8420.18374330057086993No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8320.1815610760985318No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8260.18025174141512892No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7380.16104816605855343No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT7270.1586477191389815No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7130.15559260487770812No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7100.1549379375360067No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6930.1512281559330319No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6690.1459908171994204No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6650.14511792741048515No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6040.13180635812922262No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5910.12896946631518305No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5800.1265690193956111No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5630.12285923779263631No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA5500.12002234597859673No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5410.11805834395349243No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5210.11369389500881619No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5140.11216633787817949No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5100.11129344808924425No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT4930.10758366648626944No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG4920.10736544403903564No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4840.10561966446116514No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC4610.10060054817478746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)