FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007925750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458248
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT64941.417136572336377No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA39180.8549955482620765No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC30080.6564131212793073No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA18870.4117857579302038No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA17870.3899635132068225No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17100.37316038476981894No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16570.36159459506642694No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15100.32951589532305653No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14080.30725720570520765No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13890.30311097920776525No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12100.2640491611529128No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11920.2601211571027042No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10820.23611668790698487No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10430.2276060124648662No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9650.2105846615806288No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9410.20534732284701734No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9360.20425621061084825No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8270.18046996386236275No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7710.16824950681726925No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7590.1656308374504635No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7590.1656308374504635No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA7570.16519439255599588No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7170.1564654946666434No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6850.1494823763551614No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6850.1494823763551614No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6210.1355161397321974No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6160.13442502749602833No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5880.1283147989734816No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5870.12809657652624779No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5860.127878354079014No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5700.12438679492327298No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5570.12154990310923343No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA5400.11784012150625861No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5390.1176218990590248No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5210.11369389500881619No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC4940.10780188893350325No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4700.10256455019989176No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC4670.10190988285819032No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG4650.1014734379637227No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)