FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007925751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433853
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA25000.5762320417284196No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA22710.5234491867060963No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC19420.44761705001463636No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT17020.39229877400870805No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC15510.3574943586883115No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA15020.34620021067043444No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT13940.32130698646776673No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13500.31116530253334657No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12870.2966442550817904No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12720.29318686283141987No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC12370.285119614247222No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT10250.236255137108652No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9630.2219645824737872No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9590.22104261120702173No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8560.19730185108781084No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8210.18923460250361296No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8160.18808213842015614No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC7700.17747946885235322No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7340.16918172745146398No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT7050.1624974357674143No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6450.14866786676593224No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6440.14843737394924086No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6360.14659343141570993No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6140.14152258944849985No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6060.1396786469149689No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5990.13806519719812932No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5940.13691273311467247No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5710.131611398330771No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA5150.11870380059605443No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5100.11755133651259758No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT5060.11662936524583212No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5050.11639887242914074No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5030.11593788679575802No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5000.1152464083456839No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG4860.11201950891200475No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG4820.11109753764523929No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4810.11086704482854792No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG4650.10717915976148602No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC4520.10418275314449826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)