FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007925753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462811
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA26780.5786379321148374No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA25090.5421219461075903No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC21740.4697381868624557No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT18100.3910883708468467No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC16570.3580295196095166No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT15860.3426884840680105No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA15620.33750278191313515No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA15220.328859944988343No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14330.30962963283068035No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC13200.2852136185181424No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA13010.2811082709788661No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11210.24221550481730125No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11040.23854229912426456No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG9520.2056995188100542No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9250.1998656038858195No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9080.1961923981927828No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8730.18862991588358963No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC8400.18149957542063608No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8020.17328888034208348No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7420.16032462495489522No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6770.14628001495210788No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6750.14584787310586828No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6750.14584787310586828No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT6570.1419585964897118No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6390.13806931987355528No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6350.13720503618107607No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6060.13093897941060173No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5780.1248889935632472No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5720.12359256802452837No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5710.12337649710140856No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA5620.12143185879333032No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5490.11862293679277286No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5280.11408544740725696No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT5200.11235688002229852No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5140.1110604544835797No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5050.10911581617550144No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG4980.10760331971366281No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4930.10652296509806379No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC4790.10349797217438653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)