FastQCFastQC Report
Sun 21 May 2023
EGAF00007925781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482568
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA25160.5213772981217155No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA23600.4890502478407189No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC20930.43372125793670524No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT17970.37238275227532697No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC17080.35393975564065583No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA16290.33756900581886906No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA14240.2950879461547388No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA13650.28286168995872085No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13640.2826544652774324No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT13300.2756088261136254No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12190.2526068864906086No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT11750.2434890005139172No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC10630.2202798362096119No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9790.20287296298138296No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT9420.19520564977371066No Hit
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG9100.18857445997248057No Hit
GCGCAGACCCGGGAAACGGTCACGTGCCGCCTGCAGTGCGCGAG8790.18215049485253892No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8760.1815288208086736No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA8490.17593375441388573No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT8220.17033868801909782No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC7610.15769798246050298No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7170.1485800964838116No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6880.14257058072644685No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6850.14194890668258153No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6730.13946221050712024No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6140.12723595431110227No Hit
CGGCTGGTCTTCGGCCGTTGCTTCATCGTCGTGCAGATAATCCG6120.12682150494852537No Hit
CGGCTGGTCCTCTGCAGTAGCTTCTTCATCGGACAGGTAATCCG5970.1237131347291988No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5840.12101921387244906No Hit
TTCCATCGGCTGCTGCGGGCTTACCTGGGTCAGCTGCGGTGGCT5600.1160458215215265No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5430.11252300193962302No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5420.11231577725833457No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT5220.10817128363256577No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5190.10754960958870045No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5030.10423401468808542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)