FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007925870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412127
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT46481.1278076903478782No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36640.889046337658052No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC18910.4588391442443713No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17870.4336042045291864No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17510.42486903308931473No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14900.36153904015024496No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14580.35377444331480346No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12530.3040324948377563No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC12480.3028192765822186No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11570.2807387043314318No Hit
ATCGGCTCCCTGGCTGGTGCAATTGGCGGTA10850.2632683614516884No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10610.2574449138251073No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10540.2557464082673545No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10000.24264365110754693No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT9970.2419157201542243No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9780.23730549078318092No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA9700.23536434157432054No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9630.2336658360165677No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT8700.21109997646356582No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG8620.20915882725470544No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8600.20867353995249038No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8400.20382066693033943No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG8040.19508549549046775No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7850.19047526611942434No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7610.18465181849284323No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7550.18319595658619794No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7390.1793136581684772No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6950.16863733751974513No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6930.16815205021753005No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6460.15674779861547533No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6450.1565051549643678No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6160.1494684890822489No Hit
GACGCCGCGTCTTCTTCTTCCGCGGGTCAGT6120.14849791447781874No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5940.1441303287578829No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG5850.14194653589791495No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5750.1395200993868395No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5120.12423354936706404No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5060.12277768746041876No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG4900.118895389042698No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4810.11671159618273008No Hit
GATGCAGCTTCCAACTCTGCAAACCGTCAGG4800.11646895253162254No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG4620.11210136681168668No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC4620.11210136681168668No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4370.10603527553399801No Hit
AAAGAGCTGTACGATTCCCCGTCTGCGCAGC4250.10312355172070746No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG4220.10239562076738482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)