FastQCFastQC Report
Wed 31 May 2023
EGAF00007925889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503081
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA55321.0996241161960003No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA50220.998248790950165No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT23450.46612772098330085No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC19910.3957613187538388No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA15880.315654934294875No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA15860.3152573839997933No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC15350.30511985147520976No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT13650.27132807639326467No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA13370.2657623722621208No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT11190.22242939009821477No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10690.21249063272117213No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10550.2097077806556002No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT9840.1955947451801996No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA8950.17790375704906367No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8780.17452457954086917No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT8300.1649833724589082No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA7940.1578274671474375No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6740.13397444944253512No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG6740.13397444944253512No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC6570.1305952719343406No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6410.12741486957368695No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6210.12343936662286988No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6200.12324059147532902No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT6010.1194638636720528No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA5990.1190663133769711No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5670.1127055086556638No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT5550.11032020688517356No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA5350.1063447039343565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)