FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007925916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478954
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC34020.710297857414282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29260.6109146181052878No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27540.5750030274306093No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA23690.49461952504833445No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC21800.4551585329697632No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC21650.45202670820162266No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21340.44555427034746553No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT20810.43448848950003555No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG20740.4330269712749032No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG20050.4186205773414566No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19960.41674148248057225No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG18520.38667596470642274No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16790.35055558571386813No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16260.3394898048664381No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14650.30587488568839594No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC13130.27413906137123817No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13040.27225996651035383No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC12870.26871056510646113No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12500.2609853973450477No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12350.25785357257690716No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG12010.25075476976912187No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10850.22653532489550146No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10590.22110682863072445No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC10180.21254650759780688No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10060.21004104778329444No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9800.20461255151851743No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9380.19584344216772384No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9040.18874463935993854No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG8950.18686554449905418No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8390.17517339869799606No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7940.16577792439357433No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7830.1634812528969379No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7660.15993185149304526No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7310.1526242603673839No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG7070.14761334073835902No Hit
GAACAGGGCCCGGCGGACGATCCGGGTGAGG7000.14615182251322675No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6770.14134969120207785No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA6750.1409321145663258No Hit
GAACAGGGTCCGGCAGACGATCCGGGTGAAG6550.13675634820880503No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6250.13049269867252386No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA5970.12464662577199481No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5900.12318510754686254No Hit
GACGACCCGGGTGAAGGCCCGTCTACTGGCC5890.12297631922898651No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5860.12234995427535839No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5750.12005328277872196No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5710.11921812950721782No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5600.11692145801058139No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5480.11441599819606893No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC5370.11211932669943252No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC5300.11065780847430025No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5250.10961386688492006No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT5030.10502052389164722No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA5030.10502052389164722No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5010.10460294725589514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)