FastQCFastQC Report
Sun 21 May 2023
EGAF00007925918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446391
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65251.461723018609246No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC30750.688857974287116No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG25060.5613912466873212No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA21720.48656894964280195No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC20490.4590146306713173No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC19910.4460215371725684No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT19000.4256358215107383No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG18660.41801918049423037No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG18640.41757114278737695No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18310.4101785206242958No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17860.400097672220094No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG17140.38396831477337134No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14590.32684350714956173No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14220.31855480957277366No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13770.30847396116857195No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12730.28517600041219465No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC12690.28427992499848787No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC12300.2755431897148464No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11670.26143000194896404No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11500.25762168144071007No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG10600.23745998463230666No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC10230.22917128705551862No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10120.22670707966782486No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9390.2103537033676754No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9260.20744145827312827No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA9090.20363313776487427No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8190.18347144095647092No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG8060.18055919586192373No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7790.17451068681940274No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7210.16151759332065388No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7080.1586053482261067No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6890.1543489900109993No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6840.1532288957438658No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6630.14852449982190502No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA6290.14090785880539705No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6230.13956374568483684No Hit
GAACAGGGCCCGGCGGACGATCCGGGTGAGG6200.1388916891245567No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG6110.13687551944371637No Hit
GAACAGGGTCCGGCAGACGATCCGGGTGAAG5730.12836280301350161No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA5680.1272427087463681No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5630.12612261447923456No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5420.12141821855727378No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5410.12119419970384707No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5320.11917803002300673No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5290.11850597346272662No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5200.11648980378188628No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA5170.11581774722160616No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC5110.11447363410104594No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5090.11402559639419253No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4930.11044129473936526No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT4870.10909718161880504No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC4800.1075290496448181No Hit
GACGACCCGGGTGAAGGCCCGTCTACTGGCC4700.10528886111055107No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4670.10461680455027096No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)