FastQCFastQC Report
Wed 31 May 2023
EGAF00007925944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409715
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63751.5559596304748422No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA26330.6426418363984722No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC19960.48716790940043686No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT18560.45299781555471486No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG18390.44884858987344867No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG18000.43932977801642603No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG17670.43127539875279164No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG16630.4058919004673981No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG15180.37050144612718594No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG14500.35390454340212096No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12640.3085071330070903No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA12290.2999646095456598No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11560.2821473463261047No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA11140.27189631817238813No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11090.27067595767789804No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10780.2631097226120596No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG8920.2177123122170289No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT8830.21551566332694677No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8590.20965793295339444No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8400.20502056307433214No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7840.19135252553604334No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG7690.18769144405257313No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7220.17622005540436644No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC7200.17573191120657042No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7000.17085046922861014No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6760.16499273885505777No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6730.16426052255836374No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6730.16426052255836374No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6580.16059944107489352No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6350.1549857828002392No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5890.14375846625093053No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5810.1418058894597464No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5670.1383888800751742No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5570.13594815908619406No Hit
CCGGCAGTTGCGCCGACCAGCGCGGCTAGCC5160.12594120303137546No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5040.12301233784459929No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4970.12130383315231319No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG4960.12105976105341518No Hit
GAAACTCTGTATCAGAAAACCGACGCAGGTA4910.1198394005589251No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4890.11935125636112907No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG4690.11446981438316879No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT4640.11324945388867871No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4560.1112968770974946No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC4560.1112968770974946No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4520.11032058870190253No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT4200.10251028153716607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)