FastQCFastQC Report
Sun 21 May 2023
EGAF00007925957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007925957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481441
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG42010.8725887491925284No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG33510.6960354435953731No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG30830.6403692248894465No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG30030.6237524431861848No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG23140.4806404107668437No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA19590.4069034419586201No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT18520.38467849643050755No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG18510.3844707866592168No Hit
CTGGCCGTCGATGTTACGGACCGGAATCAGCAGTGCACCATCAA18370.381562849861146No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG17990.3736698785520967No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG17630.36619232678562896No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA16890.35082180371011196No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG15070.31301862533519165No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC13870.28809345278029913No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG13660.28373154758319297No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG13250.27521544696027134No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG12480.25922179457088196No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG12010.24945943532021578No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG11850.24613607897956344No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11840.24592836920827266No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG11740.24385127149536495No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT11340.2355428806437341No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG11020.22889616796242948No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG10900.2264036507069402No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG10770.2237034236801602No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10640.22100319665338017No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG10180.2114485471740047No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG9630.2000245097530123No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT9250.19213153844396302No Hit
GGCTGGAATGGATTCGGAGTTGCGCGGGCCGGTCGGCAGGGTGT9070.18839276256072915No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8980.18652337461911223No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8410.17468391765553828No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA8220.17073743200101363No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8020.1665832365751982No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA7960.16533697794745358No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT7770.16139049229292893No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT7690.15972881412260276No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7240.15038187441451809No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6920.1437351617332134No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6670.13854241745094414No Hit
CAGGAAGTTTTCTACAGAGGATTCAGTGCGAGAGTGGTAGTTTT6410.1331419633973841No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT6350.13189570476963947No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5840.12130250643381016No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT5840.12130250643381016No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG5760.11964082826348399No Hit
GTTGCGTGGGCCGGTCGGCAGGGTATCTGCTACACGAGCTACCG5640.11714831100799473No Hit
TGGCTGTTCGTTATCTACCTCGATGATTGCAACGCACGCACCAC5480.11382495466734242No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4840.10053152930473308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)